Pelatihan Uji in Vivo Hewan Laboratorium Berbasis Penelitian Bidang Bioteknologi Kesehatan pada Siswa SMA Pangudi Luhur Yogyakarta

Vinsa Prakasita, Nadya Pangaribuan, Enjelin Susilowati, Yoga Kristiawan

Diabetes mellitus (DM) is one of the non-communicable diseases with a high incidence rate in Indonesia. DM is a chronic metabolic disorder caused by disruptions in insulin secretion, characterized by elevated blood glucose levels. The treatment of diabetes using available commercial drugs has limitations such as being less effective, costly, and having potential side effects, thus necessitating an alternative treatment solution for DM using natural ingredients. To understand the effects of a product, the use of animal testing serves as an in vivo testing method. In vivo testing is conducted in basic and applied research, drug testing, testing of biological substances, and serves as an educational tool. Among various types of experimental animals, approximately 40% of the animals used for testing are mice (Mus musculus). The purpose of conducting animal testing is to ensure that the raw materials used in a product are truly safe and that the compounds within the product do not have negative physiological effects on body tissues. Therefore, introducing knowledge and the application of health biotechnology to high school students is important to enhance their interest and critical thinking towards a problem in a mini research setting. This training employs the service-learning method, where the speakers provide knowledge transfer to high school students that connects classroom learning with the health needs and issues present in the community. The students engage in activities involving in vivo testing on experimental animals. These activities include theoretical exposure to the material, practical handling, and laboratory animal restraint. The students are capable of inducing DM intraperitoneally, providing oral treatment with natural extract substances, obtaining blood samples, and checking blood glucose levels using a glucometer. The students are also capable of analyzing the results of blood glucose levels in mice, with 50% of students scoring above 75 on the quiz (range 0-100). This demonstrates a good understanding of students after receiving training in in vivo tests on laboratory animals. However, further training is needed for students interested in the direction of in vivo testing research to enhance both their soft and hard skills.

high school students, in vivo diabetes testing training, laboratory animals

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